A DANDENONG woman was killed when a semi-trailer collided with two oncoming vehicles in Dixons Creek last night.
Police believe the semi-trailer jacknifed, striking the other vehicles driving in the opposite direction on the Melba Highway about 8.20pm.
The woman, in her 50s, was driving alone in a Mitsubishi van and died at the scene.
A 29-year-old woman of Pakenham Upper and a 21-year-old Berwick woman in a Nissan Navara towing a horse float were taken to Royal Melbourne Hospital with non-life-threatening injuries.
A horse died at the scene, another was euthanised by a ranger.
The truck driver, a 41-year-old Swan Hill man was taken to The Alfred hospital in a serious condition.
Major collision unit detectives are investigating the crash.
If you have information on the crash, call Crime Stoppers on 1800 333 000 or visit crimestoppers.com.au